Frequency of micronuclei and apoptosis in exfoliated buccal cells in chronic in flammation of oral mucosa

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Muthanna Journal of Pure Sciences – MJPS

Vol.3 – No. 2 / October 2016

Fadil A. AL-Quraishe


Chronic inflammations of the oral mucosa (CIOM) induced by biological , chemical , and physical factors and are in turn associated with an increased risk of oral cancers. The objective of the current study was the investigate the spontaneous genetic damageusingthe micronuclei (MN) and apoptosis test on exfoliated cells from (CIOM) patients. Cytological tests included, in addition to (MN),counting apoptosis(karyorrhexis, condensed chromatin , and, pyknosis). The study sample comprised 42 individuals aged (20–40) years including twenty two patients (13 males and 9 females ) revealed (CIOM) and the another 20 (12 males and 8 females) were healthy control group. MN frequency was significantly increased (P<0.001) in buccal mucosa cells obtained from lesion area (LA) and normal area (NA) from chronic inflammation patients with mean value (7.53 ± 1.49) and (5.02 ± 1.47) respectively, when compared with the healthy control group ( 2.40 ± 1.40). Lower frequency of apoptosis observed in (LA) from chronic inflammation patients compared with control group, was (P=0.06) more over apoptosis was significant increased (P<0.001) in NA of chronic inflammation patients when compared with cells of control group. The MN was regarded with the gender, age, mouthwashes uses , and , oral hygiene of the patients. The frequency of MN was significantly higher in the females than males in both patients and control groups (P< 0.01), whereas non-significant differences in MN occurrence was observed in relation to age.
However MN frequency was significantly higher in poor oral hygiene and mouthwash users (P<0.01) patients group but shown non-significant differences in poor and good oral hygiene in control group . Also the significantly higher frequency was increased MN in mouthwashes user in control group, when compared with non-users. The results suggest that increased MN and decreased apoptosis in the long periods chronic inflammation of the oral mucosa patients could be consider as a useful diagnostic bio-monitoring assay to prevent the risk from transformation of chronic inflammation to pre cancers lesions.
Key Words :Micronuclei ,Apoptosis, Chronic inflammation, Oral mucosa exfoliated.



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