First isolation of G. lamblia genotyping by Multiplex-PCR assay for detection of mixed infection in Human Stool in AL-Muthanna province–Iraq

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Muthanna Journal of Pure Sciences – MJPS

Vol.3 – No. 2 / October 2016

Nabaa Munther Turki, Muhammed Oda Mallah, and Yassir Dakheel Kremsh Alasadiy

Abstract

This study was carried out in AL-Muthanna Province during the period from October 2014 to March 2015 for detect the mixed genotyping of G. lamblia. A total of 200 stool samples (male and female), were taken from human aged (>1-40>) years who suffering from acute or persistent diarrhea. The samples examined by Multiplex-PCR assay, the results of the PCR assay showed the presence of mixed Assemblages of G. lamblia in 17 positive samples and high percentage of mixed assemblages EF was 41.18% and lower percentage in assemblages ABEF, AE, BE, ABF, ABE were 5.88% with significant differences at level P≤0.05.
Keyword: Giardia lamblia ,Genotyping ,Human ,Stool ,Iraq .

 

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