Detection of Pork in Canned Meat using TaqMan Real-time PCR

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Muthanna Journal of Pure Sciences – MJPS

Vol.3 – No. 2 / October 2016

Nihad A.M. Al-Rashedi Emad Uldeen Hateem
Biology Department – College of Science – Muthanna University

Abstract

This study was aimed to detection of pork contamination in commercial canned meat product in Iraq market using real-time PCR method through analysis of mitochondrial cytochrome b gene. Method based on porcine-specific primers and TaqMan probe were designed to amplify 130 bp for assessing pork adulteration in canned meat. Testing the twenty samples of local market that show no pork detection to have pork contamination in nineteen meat cans. In conclusion, specific PCR amplification of cytochrome b gene is useful in detection of hidden pork meat mince in processed foods because of its rapid, specificity and sensitivity. This method will ensure that trust of the customers with these products.
Key words: Real-time PCR; cytochrome b; canned meat; pork.

 

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