Molecular detection of some genes encoded to virulence Stx1, Stx2, eaeA and hlyA in E.coli isolated from infants children Suffering Frequent diarrhea and evaluate Saccharomyces boulardii Filtrates in inhibited

Muthanna Journal of Pure Sciences – MJPS

VOL.4 – NO. 1 / January 2017

Rahem E. Kh. Al-Zaidi

Al-Muthanna University/ Education for Pure Science College/ Biology Department

Abstract

This study was aimed to detect some virulence genes of Escherichia coli which isolated from Infants whose suffered from Chronic Diarrhea and evaluated the culture filtrate antimicrobial of probiotic isolate Saccharomyces boulardii. Fifty two swabs were collected from Infants feces in Female and Children Educational Hospital in Samawa city-Iraq from children suffered from frequent diarrhea after specialist doctor described that, isolates were identified by cultural ,microscopic and traditional biochemical tests, then confirmed by API 20E system. eight E.coli isolates were identified from 52 swabs. Antibiotic susceptibility for eight isolates was performed by disk diffusion methods to standard antibiotic which were containing ampicillin, cephalexin, chloramphenicol, gentamicin , tetracycline and erythromycin. Results showed some isolates have Multi-resistance to used antibiotics. DNA extraction and multiplex Polymerase Chain Reaction (mPCR) was detected for some virulence genes stx1, stx2, eae A and hly A genes by using specific primers for specific sequences for this genes, Results showed four bands were appeared in three isolates. Thin antimicrobial activity of Saccharomyces boulardii supernatant was tested, results showed that supernatant have antimicrobial activity against isolates with varied inhibition zone, the diameter inhibition zone ranged 9-14mm.
Keywords : Escherichia coli. virulence, infants, Frequent diarrhea , Saccharomyces boulardii

 

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